Leptospirosis is a deadly bacterial infection increasingly affecting many domestic pets in Maryland. Dogs typically encounter leptospira bacteria when swimming in or drinking contaminated water near ponds, marshes or wooded areas. Leptospirosis is a zoonotic disease, which means it can spread from canines to humans, including young children who are in close contact with their pets. Without prompt treatment, Leptospirosis can cause serious health problems, including death. In order to prevent this deadly bacterial infection, our Solomons team of veterinarians strongly recommends vaccinating dogs against Leptospirosis. Leptospirosis Vaccination Essential to Canine Health Leptospirosis infections primarily occur in marshy or muddy areas with stagnant surface water. Raccoons, opossums, rodents can be infected and shed the bacteria in their urine. Hunting and sporting dogs are at greatest risk for this bacterial infection, although any canines that live or play near water, farms or wooded areas are susceptible to infection because of their exposure to wildlife. Dogs that spend time in boarding kennels are also at elevated risk for infection. Leptospirosis infections occur when the spiral-shaped bacteria penetrate the skin and spread throughout the body via the bloodstream. Once a pet is infected, these bacteria can reproduce in the pet’s kidneys, liver, central nervous system, reproductive system and eyes. Depending on the strength of your pet’s immune system, your pet may be able to partially fight off this infection. However, without pre-existing immunization protection, fighting off the bacterial infection in the kidneys and liver can be difficult. If the infection progresses in these organs, it can be fatal. Young children who are in close contact with the family pet – and also have weaker immune systems than adults – are at greatest risk for this infection. This.
Nucleic acid testing ( NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and an the internal extraction template ( DNA or RNA controls for the quality of the nucleic acid extraction and eliminates false negative results. The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above. Packaged, optimised and ready to use. Expect Better Data. Advanced kit contents: Primer and probe mix (150 reactions) Reverse Transcription, target specific primers ( RNA genome viruses only) Copy number standard curve (sufficient for multiple standard curves) Internal extraction control - Read through VIC channel* (150 tests) Endogenous control (150 tests) RNAse/ DNAse free water *alternative fluorophores available on request Standard kit contents: Primer and probe mix (150 reactions) Reverse Transcription, target specific primers ( RNA genome viruses only) Copy number standard curve (sufficient for multiple standard curves) RNAse/ DNAse free water The kit includes lyophilised Master Mix as well as the nucleic acid testing components themselves. Simply follow the easy 3 step protocol: Re-suspend the kit contents Combine the kit contents with your DNA/ RNA Load your sample in to the genesig q16 DNA/ RNA extraction: genesig® Easy DNA/ RNA.
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